Workshop > Bruker workshop

Comprehensive Understanding of Material Behavior at the Micro and Nanoscale

Workshop program :

10:00 -10:10    Presentation of Bruker's products – Jérôme Beaumale

10:10 – 10:55 - Nanoscale Mechanobiology by AFM – from Single Molecules via Living Cells towards Tissues - TBA Applications Scientist, Bruker Nano Systems and Metrology, Berlin, Germany

In recent years Atomic Force Microscopy (AFM) has emerged as a versatile tool no longer limited to materials research.  Evolution in design and usability made it a powerful tool for life science research. Here, we present our multipurpose AFM platform allowing comprehensive characterization of biological samples, such as, live cells, tissues and biomaterials at the nanoscale.

The nano-mechanical analysis of cells and tissues is increasingly gaining in importance in different fields of cell biology, such as, cancer research and developmental biology. Here, we will show how nanomechanical data can be obtained on biologically relevant samples by different approaches. Using single cell force spectroscopy (SCFS), cell-substrate or cell-cell/tissue interactions can be investigated and quantified down to single protein unbinding events. AFM based SCFS turned into a well-established technique giving insights into the early cell adhesion process.

With our Quantitative Imaging (QI™) mode several sample properties, such as the topography, stiffness and adhesion, can be obtained with one measurement at high resolution. Even more complex data like Young´s modulus images or recognition events can be extracted. A variety of biological samples will be discussed to demonstrate the capability and flexibility of QI™.

The instrument demonstration will introduce the relevant AFM basics and the workflow from sample preparation to AFM measurements and data analysis. 

Remote demonstration from Berlin of the NanoWizard4 Atomique Force Microscopy – mechanical study biological object.

11:00- 11:45 Nano-indentation for mechanical characterization of biological tissues

Bruker takes great pride in offering the most comprehensive suite of quantitative nanomechanical and nanotribological test instruments in the market. Our industry-leading technologies are specifically designed to enable new frontiers in nanoscale materials characterization, materials development, and process monitoring. Bruker’s quantitative nanoscale testing solutions are routinely utilized in diverse areas of research and development to help overcome today’s most complex material challenges. Highly customizable and upgradable, Bruker’s Hysitron nanomechanical test instruments will keep pace with your evolving characterization needs and provide new insights into nanoscale material behavior